![]() We conclude that UNCL is a novel inner nuclear membrane protein that associates with RNA and is involved in the cell-surface expression of neuronal nicotinic receptors. When UNCL was co-expressed with neural nicotinic receptors in Xenopus oocytes or COS cells it increased expression of functional cell surface receptors up to 1.6-fold. Since unc-50 is required for expression of assembled muscle-type nicotinic receptors in the nematode we investigated whether UNCL had a similar function for mammalian nicotinic receptors. Homopolymer RNA-binding assays performed on in vitro translated UNCL protein and ‘structural modeling by homology’ suggest that UNCL binds RNA via an amino-terminal RNA Recognition-like Motif. UNCL-HA was extractable in 350 mM NaCl, suggesting that UNCL is not associated with the nuclear matrix. Hemagglutinin tagged UNCL was stably transfected into SaOS-2 osteosarcoma cells and exhibited a nuclear rim staining pattern which was retained following extraction with 1% Triton X-100, suggesting that UNCL localizes to the inner nuclear membrane. Hydropathy plot analysis and in vitro translation experiments with microsomal membranes indicate that UNCL is a transmembrane protein. The 777 kb rat UNCL cDNA encodes a 259 amino acid protein that is expressed in a wide variety of tissues with highest mRNA levels in brain, kidney and testis. elegans gene unc-50 that we have named UNCL. This is a PLOS Computational Biology Software paper.We isolated a mammalian homologue of the C. ReproPhylo is a platform independent CC0 Python module and is easily installed as a Docker image or a WinPython self-sufficient package, with a Jupyter Notebook GUI, or as a slimmer version in a Galaxy distribution. The system facilitates thorough experimental exploration of both parameters and data. ![]() In addition, ReproPhylo produces an extensive human-readable report and generates a comprehensive experimental archive file, both of which are suitable for submission with publications. This file, along with a Git repository, are the primary reproducibility out-puts of the program. This ‘single file ’ approach ensures the persistence of prove-nance across iterations of the analysis, with changes automatically managed by the version control program Git. Reproducibility, and instantaneous repeatability, is built in to the Repro-Phylo system and does not require user intervention or configuration because it stores the experimental workflow as a single, serialized Python object containing explicit provenance and environment information. We pres-ent ReproPhylo, a phylogenomic analysis environment developed to ensure experimental reproducibility, to facilitate the handling of large-scale data, and to assist methodological experimentation. The reproducibility of experiments is key to the scientific process, and particularly neces-sary for accurate reporting of analyses in data-rich fields such as phylogenomics. In combination with morphometric data and geographic distributions, several morphological characters such as degree of tympa-num exposure, skin texture, ulnar/tarsal tubercles and sexual secondary characters (vocal nov.- originally confused with Pristimantis acuminatus. Molecu-lar phylogenetic analyses revealed a close relationship between three new species-Pristi-mantis enigmaticus sp. We used a combination of DNA sequences from three mitochondrial genes, morphometric data, and comparisons of ecological niche models (ENMs) to infer a phylogenetic hypothesis for the Pristimantis acuminatus complex. Robber frogs (Pristimantis) are exem-plary, as many of the species show high morphological variation within populations, but few traits that are diagnostic of species. Although species delimitation has tradition-ally been based primarily on morphological differences, the integration of new methods allows diverse lines of evidence to solve the problem. Pluralistic approaches to taxonomy facilitate a more complete appraisal of biodiversity, especially the diversification of cryptic species.
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